multisite gateway tol2 kit compatible pme Search Results


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Thermo Fisher lr clonase ii plus enzyme mix

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Image Search Results


Journal: eLife

Article Title: Basement membranes are crucial for proper olfactory placode shape, position and boundary with the brain, and for olfactory axon development

doi: 10.7554/eLife.92004

Figure Lengend Snippet:

Article Snippet: To generate the Tg(UAS:lyn-tagRFP ) line, a 10XUAS:lyn-tagRFP plasmid was synthesised by Gateway cloning (Multisite Gateway system kit, Invitrogen, 12537–023, final destination vector: pDest:tol2) and co-injected with Tol2 mRNA (25 ng/µL of plasmid and 25 ng/µL of Tol2 mRNA) in 1 cell-stage wild type embryos.

Techniques: Recombinant, Plasmid Preparation, Clone Assay, Sequencing, Amplification, Software